Fluorometric assay for determining epoxide hydrolase activity

We have developed a rapid screening procedure that enables the screening of hundreds of enzyme samples or variants for epoxide hydrolase activity towards any substrate. The procedure detects the products of the enzymatic reaction via periodate cleavage and remaining fluorescence of carboxyfluorescei...

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Veröffentlicht in:	Biotechnology letters 2004-05, Vol.26 (10), p.835-839
Hauptverfasser:	DODERER, Kai, SCHMID, Rolf D
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Biological and medical sciences Biotechnology Enzyme Activation Enzymes Epoxide hydrolase Epoxide Hydrolases - analysis Epoxide Hydrolases - chemistry Fluoresceins Fluorescence Fluorometry - methods Fundamental and applied biological sciences. Psychology Microbiology Q1 Streptomyces antibioticus - enzymology
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Beschreibung
Zusammenfassung:	We have developed a rapid screening procedure that enables the screening of hundreds of enzyme samples or variants for epoxide hydrolase activity towards any substrate. The procedure detects the products of the enzymatic reaction via periodate cleavage and remaining fluorescence of carboxyfluorescein.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/B:BILE.0000025887.36874.33