Isolation and characterization of cloned fragments of bacteriophage P1 DNA

Isolation and characterization of cloned fragments of bacteriophage P1 DNA

Fragments of PI DNA generated by endo·R· EcoRI and endo·R· BamHI were mixed with appropriate cloning vectors (ColElAp r, pBR313, pBR322) ligated in vitro and used to transform a nonsuppressing strain of Escherichia coli. In this way clones of 9 of the 26 EcoRI fragments and 5 of the 14 BamHI fragmen...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 1979-03, Vol.93 (2), p.387-397
Hauptverfasser: Mural, Richard J., Chesney, Robert H., Vapnek, Daniel, Kropf, Martha M., Scott, June Rothman
Format: Artikel
Sprache:eng
Schlagworte:
Coliphages - genetics
DNA Restriction Enzymes
DNA, Recombinant
DNA, Viral - genetics
Genes, Viral
Plasmids
Transformation, Bacterial
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Zusammenfassung:Fragments of PI DNA generated by endo·R· EcoRI and endo·R· BamHI were mixed with appropriate cloning vectors (ColElAp r, pBR313, pBR322) ligated in vitro and used to transform a nonsuppressing strain of Escherichia coli. In this way clones of 9 of the 26 EcoRI fragments and 5 of the 14 BamHI fragments were obtained. Marker rescue tests were used to ascertain which regions of the P1 genome were contained on the various DNA fragments. These cloned fragments are useful as probes for specific regions of the P1 genome.
ISSN:0042-6822
1096-0341
DOI:10.1016/0042-6822(79)90243-5