Comparison of Newcastle Virus in Hamsters Exposed by Intracerebral Injection and Intranasal Instillation

Summary Infected hamster brains from the 300th intracerebral passage and from the 4th intranasal passage titred lO-4.77, whereas infected brains from the 7th intranasal passage titred 10-3.66. Positive Newcastle chicken serum neutralized the virus from the 245th and 300th hamster passage, whereas normal chicken serum had no effect. Nasal instillation trials were conducted from the 16th and 300th hamster passage but were not successful (except for the 1st passage) until after the 245th passage. The incubation period of hamsters infected by intranasal instillation is longer than by intracerebral injection. Hamster-adapted Newcastle virus was carried through 9 passages intranasally, and brain material from this 9th nasal passage was neutralized by positive Newcastle chicken serum. Hamsters infected intranasally and intracerebrally showed symptoms of irritability followed by involuntary motor reactions and paralysis.