Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus

Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g-1 dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase....

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Veröffentlicht in:	Biotechnology letters 2004-04, Vol.26 (8), p.671-674
Hauptverfasser:	Hernandez-Dominguez, E, Campos-Tamayo, F, Vazquez-Flota, F
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyl-CoA C-Acetyltransferase - metabolism Alkalies Alkaloids Alkaloids - analysis Aromatic-L-amino-acid decarboxylase Aromatic-L-Amino-Acid Decarboxylases - metabolism Biological and medical sciences Biotechnology Catharanthus - metabolism Catharanthus roseus Cell culture Cell Culture Techniques Chemical synthesis Cyclopentanes - metabolism Fundamental and applied biological sciences. Psychology Jasmonic acid Mixed Function Oxygenases - metabolism Oxylipins Plant Shoots - metabolism Q1 Q2 Shoots Time Factors Vinblastine - analogs & derivatives Vinblastine - biosynthesis vindoline
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Zusammenfassung:	Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g-1 dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase. Shoot exposure to jasmonate shortened the time required for the maximal vindoline accumulation to 14 d.
ISSN:	0141-5492 1573-6776
DOI:	10.1023/B:BILE.0000023028.21985.07