Three-dimensional computed tomography for optical microscopes

A 3D optical imaging method is presented based on computed tomography techniques. The 3D optical transfer function of our system has a higher band spherically, since 3D reconstructed images are composed only of the in-focused information of objects. Experimental results are shown for a simulated flu...

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Veröffentlicht in:Optics communications 1994-05, Vol.107 (5), p.432-444
Hauptverfasser: Kikuchi, Susumu, Sonobe, Kazuo, Sidharta, Liliek S., Ohyama, Nagaaki
Format: Artikel
Sprache:eng
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Zusammenfassung:A 3D optical imaging method is presented based on computed tomography techniques. The 3D optical transfer function of our system has a higher band spherically, since 3D reconstructed images are composed only of the in-focused information of objects. Experimental results are shown for a simulated fluorescence microscope.
ISSN:0030-4018
1873-0310
DOI:10.1016/0030-4018(94)90361-1