A microplate immunoenzyme assay for anti-influenza antibodies

An enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase is described for the detection and quantitation of anti-influenza virus antibodies. Compared with complement fixation and hemagglutination inhibition tests, ELISA is far superior with respect to sensitivity and reliability. No...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of immunological methods 1979-01, Vol.26 (1), p.61-67
Hauptverfasser:	Lambre, Claude, Kasturi, Kuppuswamy Naidu
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral Complement Fixation Tests Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay Hemagglutination Inhibition Tests Lung - immunology Male Orthomyxoviridae Infections - immunology Rabbits Spleen - immunology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	An enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase is described for the detection and quantitation of anti-influenza virus antibodies. Compared with complement fixation and hemagglutination inhibition tests, ELISA is far superior with respect to sensitivity and reliability. Non-specific viral inhibitors present in sera do not affect the titer in ELISA. Its sensitivity, close to that of radioimmunoassay permits detection of small amounts of antibodies in pulmonary secretions and supernatants from in vitro spleen cell cultures.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(79)90041-3