Modified Bases Characterized in Intact DNA by Mass-Analyzed Ion Kinetic Energy Spectrometry

Modified Bases Characterized in Intact DNA by Mass-Analyzed Ion Kinetic Energy Spectrometry

Pyrolysis of DNA into a chemical ionization source yields protonated bases and other base-containing ions. Kinetic energy spectra allow the characterization of the bases 5-methylcytosine and 1-methyladenine from underivatized salmon sperm DNA. Isomeric bases are distinguishable with this technique.

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Science (American Association for the Advancement of Science) 1979-03, Vol.203 (4386), p.1249-1251
Hauptverfasser: Schoen, A. E., Cooks, R. G., Wiebers, J. L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chemical bases
Deoxyadenine Nucleotides - analysis
Deoxycytosine Nucleotides - analysis
DNA
DNA - analysis
Ionization
Ions
Isomerism
Kinetic energy
Light beams
Male
Mass spectrometers
Mass Spectrometry
Mass spectroscopy
Methylation
Pyrolysis
Salmon
Spermatozoa
Spermatozoa - analysis
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Pyrolysis of DNA into a chemical ionization source yields protonated bases and other base-containing ions. Kinetic energy spectra allow the characterization of the bases 5-methylcytosine and 1-methyladenine from underivatized salmon sperm DNA. Isomeric bases are distinguishable with this technique.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.570725