Mouse nerve growth factor: A rapid isolation procedure for the α and γ subunits

A rapid method for isolating the α and γ subunits of mouse submaxillary gland nerve growth factor, as by-products of the commonly used Bocchini-Angeletti (2.5 S) procedure, has been devised. Approximately 40 mg of each subunit is obtained from 400 pairs of adult glands. The subunits isolated in this...

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Veröffentlicht in:Analytical biochemistry 1979-01, Vol.92 (2), p.482-488
Hauptverfasser: Jeng, Ingming, Andres, Roger Y., Bradshaw, Ralph A.
Format: Artikel
Sprache:eng
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Zusammenfassung:A rapid method for isolating the α and γ subunits of mouse submaxillary gland nerve growth factor, as by-products of the commonly used Bocchini-Angeletti (2.5 S) procedure, has been devised. Approximately 40 mg of each subunit is obtained from 400 pairs of adult glands. The subunits isolated in this fashion are indistinguishable from those obtained from the homogeneous 7 S complex as judged by gel electrophoresis or their association-dissociation behavior.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(79)90688-2