Oxidative and Conjugative Xenobiotic Metabolism in Zebrafish Larvae In Vivo

As zebrafish larvae are being increasingly applied to toxicity testing, there is a need to understand the potential for xenobiotic metabolism by these early life-stage organisms. The expression of genes similar to mammalian cytochromes P450 (CYP) 2B6 , CYP3A5 , and UDP-glucuronosyl transferase ( UGT...

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Veröffentlicht in:	Zebrafish 2010-03, Vol.7 (1), p.23-30
Hauptverfasser:	Jones, Huw S., Panter, Grace H., Hutchinson, Thomas H., Chipman, J. Kevin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chemical Biology Cytochrome P-450 Enzyme System - genetics Danio rerio Freshwater Gene expression Gene Expression Regulation, Developmental Genetic aspects Glucuronosyltransferase - genetics Health aspects Humans Larva - metabolism Larval development Oxidants - metabolism Physiological aspects Xenobiotics Xenobiotics - metabolism Zebrafish - metabolism
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Beschreibung
Zusammenfassung:	As zebrafish larvae are being increasingly applied to toxicity testing, there is a need to understand the potential for xenobiotic metabolism by these early life-stage organisms. The expression of genes similar to mammalian cytochromes P450 (CYP) 2B6 , CYP3A5 , and UDP-glucuronosyl transferase ( UGT ) 1A1 , as well as the zebrafish CYP1A was assessed across embryonic development. Activities toward 7-ethoxyresorufin O -deethylase (EROD assay), 7-ethoxycoumarin O -deethylase (ECOD assay), and octyloxymethylresorufin (OOMR assay) were detected at 96 h postfertilization, as was significant phenolic conjugation in the EROD assay ( p
ISSN:	1545-8547 1557-8542
DOI:	10.1089/zeb.2009.0630