Application of RAPD-PCR for the initial identification of gynogenetic clones in goldfish (Carassius auratus)

In teleost, genetically clonal lines could be induced by continuous gynogenetic treatments, inhibition of first cleavage in the first generation and subsequent suppression of polar body release in the second one. And to identify the genetic homogeneity of these prospective clonal lines, scale transplantations are performed in goldfish. In this study, we tried to apply RAPD-PCR as an initial identification method. First, we tried screening of optimal primers for genetic identification in the first generation of six gynogens induced by first cleavage inhibition, and found 5 primers which show polymorphic appearance. Then, the DNA amplifications by these 5 primers were carried out to two prospective clonal lines A-G2f and B-G2. While polymorphic appearance was observed in B-G2 lien, monomorphic appearance was observed in A-G2f line. Furthermore, transplanted donor scales were accepted between individuals of A-G2f line. These results suggest that RAPD-PCR is useful as an initial method to identify the genetic homogeneity of the prospective clonal lines in goldfish.