improved method for the enzymatic transformation of nucleosides into 5'-monophosphates

An improved method to transform nucleosides into 5-monophosphates using nucleoside phosphotransferase from Erwinia herbicola is reported. The method is based on the shift in the equilibrium state of the reaction to the formation of desired product due to its precipitation by Zn2+. Under optimal cond...

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Veröffentlicht in:	Biotechnology letters 2004-12, Vol.26 (24), p.1847-1850
Hauptverfasser:	Barai, V.N, Kvach, S.V, Zinchenko, A.I, Mikhailopulo, I.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Enzyme Activation Enzymes Erwinia - enzymology Erwinia herbicola Fundamental and applied biological sciences. Psychology Nucleoside phosphotransferase nucleosides Nucleosides - chemistry Pantoea agglomerans Phosphates Phosphates - chemistry Phosphotransferases - chemistry Q1 Q2 Solubility Transformation Zinc Zinc - chemistry
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Beschreibung
Zusammenfassung:	An improved method to transform nucleosides into 5-monophosphates using nucleoside phosphotransferase from Erwinia herbicola is reported. The method is based on the shift in the equilibrium state of the reaction to the formation of desired product due to its precipitation by Zn2+. Under optimal conditions, the extent of nucleoside transformations into nucleoside-5-monophosphates were 41-91% (mol).
ISSN:	0141-5492 1573-6776
DOI:	10.1007/s10529-004-5311-4