Coupling of gamma-globulin to microcrytstalline cellulose by periodate oxidation

Anti-rabbit IgG sheep gamma-globulins were covalently coupled to periodate oxidized microcrystalline cellulose using the Schiff reaction. Optimal conditions (sodium m-periodate concentration and gamma-globulin amount) were studied measuring the ability of this solid-phase antibody to bind glucose ox...

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Veröffentlicht in:Journal of immunological methods 1979, Vol.25 (1), p.49-53
Hauptverfasser: Ferrua, B, Maiolini, R, Masseyeff, R
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container_title Journal of immunological methods
container_volume 25
creator Ferrua, B
Maiolini, R
Masseyeff, R
description Anti-rabbit IgG sheep gamma-globulins were covalently coupled to periodate oxidized microcrystalline cellulose using the Schiff reaction. Optimal conditions (sodium m-periodate concentration and gamma-globulin amount) were studied measuring the ability of this solid-phase antibody to bind glucose oxidase-labeled rabbit IgG.
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Animals
Antigens
Binding Sites, Antibody
Cellulose
Dose-Response Relationship, Drug
gamma-Globulins - immunology
Oxidation-Reduction
Periodic Acid - pharmacology
Rabbits
Sheep
title Coupling of gamma-globulin to microcrytstalline cellulose by periodate oxidation
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