Coupling of gamma-globulin to microcrytstalline cellulose by periodate oxidation

Anti-rabbit IgG sheep gamma-globulins were covalently coupled to periodate oxidized microcrystalline cellulose using the Schiff reaction. Optimal conditions (sodium m-periodate concentration and gamma-globulin amount) were studied measuring the ability of this solid-phase antibody to bind glucose ox...

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Veröffentlicht in:	Journal of immunological methods 1979, Vol.25 (1), p.49-53
Hauptverfasser:	Ferrua, B, Maiolini, R, Masseyeff, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens Binding Sites, Antibody Cellulose Dose-Response Relationship, Drug gamma-Globulins - immunology Oxidation-Reduction Periodic Acid - pharmacology Rabbits Sheep
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Beschreibung
Zusammenfassung:	Anti-rabbit IgG sheep gamma-globulins were covalently coupled to periodate oxidized microcrystalline cellulose using the Schiff reaction. Optimal conditions (sodium m-periodate concentration and gamma-globulin amount) were studied measuring the ability of this solid-phase antibody to bind glucose oxidase-labeled rabbit IgG.
ISSN:	0022-1759