A modified orcinol test for the specific determination of RNA

A modified orcinol test for the specific determination of RNA

The standard orcinol test for estimating RNA is modified and developed as a specific method for the determination of RNA in the presence of DNA and proteins. The main differences in the procedure of the modified test when compared to that of the standard test are preincubation of the samples with H...

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Veröffentlicht in:Analytical biochemistry 1978-11, Vol.91 (1), p.130-137
Hauptverfasser: Almog, Rami, Shirey, Terry L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cattle
Chlorides
DNA - analysis
Ferric Compounds
Indicators and Reagents
Methods
Resorcinols
RNA - analysis
Spectrophotometry
Sulfuric Acids
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Zusammenfassung:The standard orcinol test for estimating RNA is modified and developed as a specific method for the determination of RNA in the presence of DNA and proteins. The main differences in the procedure of the modified test when compared to that of the standard test are preincubation of the samples with H 2SO 4 before addition of the orcinol reagent, decreased concentration of orcinol, no addition of FeCl 3·6H 2O, and quantitation of RNA at its maximum absorbance under these conditions at 500 nm where interferences from DNA and proteins were minimal.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(78)90823-0