[17] Propagation and scaling-up of suspension cultures

The general approach discussed here is one that should serve as a guide for the culture of almost any type of cell in suspension culture. Following the initiation of cells in culture, iti s advised that optimal conditions for growth be determined. In order to maintain th integrity of the cells and to protect the time invested in their growth it is also mandatory that one effect adequate quality-control measures to assure that cells are maintained in a healthy state, free from contamination, and that aliquots are stored for future reference and use. By adherence to these relatively simple rules, one will find that cells in culture can be a most valuable tool in molecular biology.