A Structural Model of Transcription Elongation

The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2000-07, Vol.289 (5479), p.619-625
Hauptverfasser: Korzheva, Nataliya, Mustaev, Arkady, Kozlov, Maxim, Malhotra, Arun, Nikiforov, Vadim, Goldfarb, Alex, Darst, Seth A.
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Sprache:eng
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Zusammenfassung:The path of the nucleic acids through a transcription elongation complex was tracked by mapping cross-links between bacterial RNA polymerase (RNAP) and transcript RNA or template DNA onto the x-ray crystal structure. In the resulting model, the downstream duplex DNA is nestled in a trough formed by the β′ subunit and enclosed on top by the β subunit. In the RNAP channel, the RNA/DNA hybrid extends from the enzyme active site, along a region of the β subunit harboring rifampicin resistance mutations, to the β′ subunit "rudder." The single-stranded RNA is then extruded through another channel formed by the β-subunit flap domain. The model provides insight into the functional properties of the transcription complex.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.289.5479.619