A Whole-Genome Assembly of Drosophila

We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portio...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2000-03, Vol.287 (5461), p.2196-2204
Hauptverfasser:	Myers, Eugene W., Sutton, Granger G., Delcher, Art L., Dew, Ian M., Fasulo, Dan P., Flanigan, Michael J., Kravitz, Saul A., Mobarry, Clark M., Knut H. J. Reinert, Remington, Karin A., Anson, Eric L., Bolanos, Randall A., Chou, Hui-Hsien, Jordan, Catherine M., Halpern, Aaron L., Lonardi, Stefano, Beasley, Ellen M., Brandon, Rhonda C., Chen, Lin, Dunn, Patrick J., Lai, Zhongwu, Liang, Yong, Nusskern, Deborah R., Zhan, Ming, Zhang, Qing, Zheng, Xiangqun, Rubin, Gerald M., Adams, Mark D., Venter, J. Craig
Format:	Artikel
Sprache:	eng
Schlagworte:	Accuracy Algorithms Animals Chromatin - genetics Chromosome mapping Chromosomes Computational Biology Computers Contig Mapping Datasets DNA sequencers Drosophila Drosophila melanogaster - genetics Euchromatin Genes, Insect Genome Genomes Genomics Heterochromatin - genetics Inferences Innovations Insects Libraries Mathematical sequences Molecular Sequence Data Physical Chromosome Mapping Pilot Projects Repetitive Sequences, Nucleic Acid Reviews Sampling Scaffolds Science and Society Sequence Analysis, DNA Sequence Tagged Sites Sequencing Shotguns Tiling
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Beschreibung
Zusammenfassung:	We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9-megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99.99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.287.5461.2196