Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum

A novel transglycosylation reaction from sucrose to L-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable L-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-alpha-D-glucopyranosyl-L-ascorbic acid by LC...

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Veröffentlicht in:	Biotechnology letters 2007-04, Vol.29 (4), p.611-615
Hauptverfasser:	KWON, Taeyeon, CHEONG TAE KIM, LEE, Jong-Hoon
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascorbic Acid - analogs & derivatives Ascorbic Acid - chemistry Bifidobacterium - enzymology Bifidobacterium - genetics Bifidobacterium longum Biological and medical sciences Biotechnology Enzyme Activation Escherichia coli - drug effects Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Glucosyltransferases - chemistry Glucosyltransferases - genetics Liquid chromatography Recombinant Proteins - chemistry Sucrose Sucrose - chemistry Vitamin C
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Beschreibung
Zusammenfassung:	A novel transglycosylation reaction from sucrose to L-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable L-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-alpha-D-glucopyranosyl-L-ascorbic acid by LC-MS/MS analysis.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/s10529-006-9285-2