Human granulocyte specific nuclear antigen(s). I: Production of antisera and determination of specificity

Non-histone protein-DNA complexes isolated from human lung tissue were used to immunize New Zealand rabbits. Quantitative microcomplement fixation and immunocytochemistry revealed a specific chromatin antigen(s) in the human granulocyte nucleus. The study demonstrates the feasibility of employing an...

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Veröffentlicht in:	Cell differentiation 1978-12, Vol.7 (6), p.313-323
Hauptverfasser:	Briggs, R.C., Chiu, J.F., Hnilica, L.S., Chytil, F., Rogers, L.W., Page, D.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibodies, Antinuclear Antibody Specificity Antigens Chromatin - immunology Chromosomal Proteins, Non-Histone - immunology DNA - immunology Granulocytes - immunology Humans Lung - immunology Organ Specificity Rabbits
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container_title	Cell differentiation
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creator	Briggs, R.C. Chiu, J.F. Hnilica, L.S. Chytil, F. Rogers, L.W. Page, D.L.
description	Non-histone protein-DNA complexes isolated from human lung tissue were used to immunize New Zealand rabbits. Quantitative microcomplement fixation and immunocytochemistry revealed a specific chromatin antigen(s) in the human granulocyte nucleus. The study demonstrates the feasibility of employing antisera against chromosomal non-histone protein-DNA complexes for the immunochemical identification of cell types.
doi_str_mv	10.1016/0045-6039(78)90032-5
format	Article
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subjects	Animals Antibodies Antibodies, Antinuclear Antibody Specificity Antigens Chromatin - immunology Chromosomal Proteins, Non-Histone - immunology DNA - immunology Granulocytes - immunology Humans Lung - immunology Organ Specificity Rabbits
title	Human granulocyte specific nuclear antigen(s). I: Production of antisera and determination of specificity
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