Human granulocyte specific nuclear antigen(s). I: Production of antisera and determination of specificity

Human granulocyte specific nuclear antigen(s). I: Production of antisera and determination of specificity

Non-histone protein-DNA complexes isolated from human lung tissue were used to immunize New Zealand rabbits. Quantitative microcomplement fixation and immunocytochemistry revealed a specific chromatin antigen(s) in the human granulocyte nucleus. The study demonstrates the feasibility of employing an...

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Veröffentlicht in:Cell differentiation 1978-12, Vol.7 (6), p.313-323
Hauptverfasser: Briggs, R.C., Chiu, J.F., Hnilica, L.S., Chytil, F., Rogers, L.W., Page, D.L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibodies
Antibodies, Antinuclear
Antibody Specificity
Antigens
Chromatin - immunology
Chromosomal Proteins, Non-Histone - immunology
DNA - immunology
Granulocytes - immunology
Humans
Lung - immunology
Organ Specificity
Rabbits
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Zusammenfassung:Non-histone protein-DNA complexes isolated from human lung tissue were used to immunize New Zealand rabbits. Quantitative microcomplement fixation and immunocytochemistry revealed a specific chromatin antigen(s) in the human granulocyte nucleus. The study demonstrates the feasibility of employing antisera against chromosomal non-histone protein-DNA complexes for the immunochemical identification of cell types.
ISSN:0045-6039
DOI:10.1016/0045-6039(78)90032-5