Reversion of B Cell Commitment upon Loss of Pax5 Expression

The transcription factor Pax5 is essential for initiating B cell lineage commitment, but its role in maintaining commitment is unknown. Using conditional Pax5 inactivation in committed pro-B cells, we demonstrate that Pax5 is required not only to initiate its B lymphoid transcription program, but al...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2002-07, Vol.297 (5578), p.110-113
Hauptverfasser: Mikkola, Ingvild, Heavey, Barry, Horcher, Markus, Busslinger, Meinrad
Format: Artikel
Sprache:eng
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Zusammenfassung:The transcription factor Pax5 is essential for initiating B cell lineage commitment, but its role in maintaining commitment is unknown. Using conditional Pax5 inactivation in committed pro-B cells, we demonstrate that Pax5 is required not only to initiate its B lymphoid transcription program, but also to maintain it in early B cell development. As a consequence of Pax5 inactivation, previously committed pro-B cells regained the capacity to differentiate into macrophages in vitro and to reconstitute T cell development in vivo in RAG2-/-mice. Hence, Pax5 expression is continuously required to maintain B cell lineage commitment, because its loss converts committed pro-B cells into hematopoietic progenitors with multilineage potential.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1067518