A NEW MICROANALYTICAL METHOD FOR DETERMINATION OF HYDROXYPROLINE IN CONNECTIVE TISSUES

A NEW MICROANALYTICAL METHOD FOR DETERMINATION OF HYDROXYPROLINE IN CONNECTIVE TISSUES

A microanalytical method for the determination of hydroxyproline in a microquantity of connective tissues (0.05-5mg) has been developed on the basis of Prockop's conventional methods. The new method is simple, rapid, sensitive, and linear in a wider assay range (0.04-20μg), with a high reproduc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Keio journal of medicine 1978, Vol.27(1), pp.43-46
Hauptverfasser: INAYAMA, SEIICHI, SHIBATA, TETSUICHI, OHTSUKI, JUN'ICHI, SAITO, SHIGEJI
Format: Artikel
Sprache:eng
Schlagworte:
Connective Tissue - analysis
Culture Techniques
Hydroxyproline - analysis
Microchemistry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A microanalytical method for the determination of hydroxyproline in a microquantity of connective tissues (0.05-5mg) has been developed on the basis of Prockop's conventional methods. The new method is simple, rapid, sensitive, and linear in a wider assay range (0.04-20μg), with a high reproducibility even when used by experts or novices. The present communication mainly describes the standard assay procedure of the method (protocol), which has been brought about by essential modification of the conventional method.
ISSN:0022-9717
1880-1293
DOI:10.2302/kjm.27.43