A PEST-Like Sequence in Listeriolysin O Essential for Listeria monocytogenes Pathogenicity

Establishment and maintenance of an intracellular niche are critical to the success of an intracellular pathogen. Here, the pore-forming protein listeriolysin O (LLO), secreted by Listeria monocytogenes, was shown to contain a PEST-like sequence (P, Pro; E, Glu; S, Ser; T, Thr) that is essential for...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 2000-11, Vol.290 (5493), p.992-995
Hauptverfasser: Decatur, Amy L., Portnoy, Daniel A.
Format: Artikel
Sprache:eng
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Zusammenfassung:Establishment and maintenance of an intracellular niche are critical to the success of an intracellular pathogen. Here, the pore-forming protein listeriolysin O (LLO), secreted by Listeria monocytogenes, was shown to contain a PEST-like sequence (P, Pro; E, Glu; S, Ser; T, Thr) that is essential for the virulence and intracellular compartmentalization of this pathogen. Mutants lacking the PEST-like sequence entered the host cytosol but subsequently permeabilized and killed the host cell. LLO lacking the PEST-like sequence accumulated in the host-cell cytosol, suggesting that this sequence targets LLO for degradation. Transfer of the sequence to perfringolysin O transformed this toxic cytolysin into a nontoxic derivative that facilitated intracellular growth.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.290.5493.992