In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines
Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortaliz...
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| Veröffentlicht in: | Biotechnology letters 2010-02, Vol.32 (2), p.195-202 |
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| creator | Yin, Jinlong Jin, Xun Beck, Samuel Kang, Dong Ho Hong, Zhongshan Li, Zhehu Jin, Yongcheng Zhang, Qiankun Choi, Yun-Jaie Kim, Sung-Chan Kim, Hyunggee |
| description | Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation. |
| doi_str_mv | 10.1007/s10529-009-0142-y |
| format | Article |
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Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-009-0142-y</identifier><identifier>PMID: 19834648</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Adipocytes - cytology ; Adipocytes - physiology ; Adipose tissue ; Animals ; Applied Microbiology ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Cattle ; Cell culture ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Line ; Cells ; Culture media ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - physiology ; Embryos ; Fibroblasts - cytology ; Fibroblasts - physiology ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic Enhancement - methods ; Life Sciences ; Microbiology ; Myoblasts - cytology ; Myoblasts - physiology ; Original Research Paper ; PPAR gamma - genetics ; PPAR gamma - metabolism ; Tissue Engineering - methods</subject><ispartof>Biotechnology letters, 2010-02, Vol.32 (2), p.195-202</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-dc3abee032888baaf22931b56afbbe037a59dd57fc12035c8541bf3da1b6996c3</citedby><cites>FETCH-LOGICAL-c487t-dc3abee032888baaf22931b56afbbe037a59dd57fc12035c8541bf3da1b6996c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-009-0142-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-009-0142-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22340950$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19834648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Jinlong</creatorcontrib><creatorcontrib>Jin, Xun</creatorcontrib><creatorcontrib>Beck, Samuel</creatorcontrib><creatorcontrib>Kang, Dong Ho</creatorcontrib><creatorcontrib>Hong, Zhongshan</creatorcontrib><creatorcontrib>Li, Zhehu</creatorcontrib><creatorcontrib>Jin, Yongcheng</creatorcontrib><creatorcontrib>Zhang, Qiankun</creatorcontrib><creatorcontrib>Choi, Yun-Jaie</creatorcontrib><creatorcontrib>Kim, Sung-Chan</creatorcontrib><creatorcontrib>Kim, Hyunggee</creatorcontrib><title>In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - physiology</subject><subject>Adipose tissue</subject><subject>Animals</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Cells</subject><subject>Culture media</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Embryos</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic Enhancement - methods</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Myoblasts - cytology</subject><subject>Myoblasts - physiology</subject><subject>Original Research Paper</subject><subject>PPAR gamma - genetics</subject><subject>PPAR gamma - metabolism</subject><subject>Tissue Engineering - methods</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1vFSEUhonR2NvqD3CjxKS6GuXwMQxL0_jRpIkL7ZrADNzQzMAV5tbMv5fJ3NjERV0Q4JznfTnkRegVkA9AiPxYgAiqGkLqAk6b5QnagZCsaaVsn6JdLUIjuKJn6LyUO1JBSeRzdAaqY7zl3Q79vo74Psw54WlJexdDj00csBnC4XQdgvcuuzgHM4cU8ZQGN-LkcW27OfRmHBfs4j5EV7EB23Rfj9hNNi9pNfDB5mRHU2bcu3HEY22XF-iZN2NxL0_7Bbr98vnn1bfm5vvX66tPN03POzk3Q8-MdY4w2nWdNcZTqhhY0RpvbS1LI9QwCOl7oISJvhMcrGeDAdsq1fbsAr3ffA85_Tq6MusplHUME106Fi05Y6KKuv-TjLXAlGKVfPcoSYEBbSWp4Nt_wLt0zLH-tzLAVacAKgQb1OdUSnZeH3KYTF40EL3GrLeYdU1PrzHrpWpen4yPdnLDg-KUawUuT4ApNSGfTexD-ctRyjhRYp2Qblyprbh3-WHCx15_s4m8SdrsczW-_UEJMAJScS4p-wPLPMq1</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Yin, Jinlong</creator><creator>Jin, Xun</creator><creator>Beck, Samuel</creator><creator>Kang, Dong Ho</creator><creator>Hong, Zhongshan</creator><creator>Li, Zhehu</creator><creator>Jin, Yongcheng</creator><creator>Zhang, Qiankun</creator><creator>Choi, Yun-Jaie</creator><creator>Kim, Sung-Chan</creator><creator>Kim, Hyunggee</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines</title><author>Yin, Jinlong ; Jin, Xun ; Beck, Samuel ; Kang, Dong Ho ; Hong, Zhongshan ; Li, Zhehu ; Jin, Yongcheng ; Zhang, Qiankun ; Choi, Yun-Jaie ; Kim, Sung-Chan ; Kim, Hyunggee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-dc3abee032888baaf22931b56afbbe037a59dd57fc12035c8541bf3da1b6996c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adipocytes - cytology</topic><topic>Adipocytes - physiology</topic><topic>Adipose tissue</topic><topic>Animals</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cattle</topic><topic>Cell culture</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Cells</topic><topic>Culture media</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Embryos</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - physiology</topic><topic>Fundamental and applied biological sciences. 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Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>19834648</pmid><doi>10.1007/s10529-009-0142-y</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2010-02, Vol.32 (2), p.195-202 |
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| subjects | Adipocytes - cytology Adipocytes - physiology Adipose tissue Animals Applied Microbiology Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Cattle Cell culture Cell Culture Techniques - methods Cell Differentiation Cell Line Cells Culture media Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology Embryos Fibroblasts - cytology Fibroblasts - physiology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic Enhancement - methods Life Sciences Microbiology Myoblasts - cytology Myoblasts - physiology Original Research Paper PPAR gamma - genetics PPAR gamma - metabolism Tissue Engineering - methods |
| title | In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines |
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