Independent regulation of calcium revealed by imaging dendritic spines

THE dendritic spine is a basic structural unit of neuronal organization. It is assumed to be a primary locus of synaptic plasticity, and to undergo long-term morphological and functional changes 1–6 , at least some of which are regulated by intracellular calcium concentrations 7–11 . It is known tha...

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Veröffentlicht in:Nature (London) 1991-11, Vol.354 (6348), p.76-80
Hauptverfasser: Guthrie, Peter B, Segal, Menahem, Kater, S. B
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Segal, Menahem
Kater, S. B
description THE dendritic spine is a basic structural unit of neuronal organization. It is assumed to be a primary locus of synaptic plasticity, and to undergo long-term morphological and functional changes 1–6 , at least some of which are regulated by intracellular calcium concentrations 7–11 . It is known that physiological stimuli can cause marked increases in intracellular calcium levels in hippocampal dendritic shafts 12,13 , but it is completely unknown to what extent such changes in the dendrites would also be seen by calcium-sensing structures within spines. Will calcium levels in all spines change in parallel with the dendrite or will there be a heterogeneous response? This study, through direct visualization and measurement of intracellular calcium concentrations in individual living spines, demonstrates that experimentally evoked changes in calcium concentrations in the dendritic shaft ([Ca 2+ ] d ) are frequently not parallelled in the spine ([Ca 2+ ] s ). This isolation is not caused by a physical diffusion barrier. This report provides, to our knowledge, the first direct demonstration of autonomous spine function.
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This study, through direct visualization and measurement of intracellular calcium concentrations in individual living spines, demonstrates that experimentally evoked changes in calcium concentrations in the dendritic shaft ([Ca 2+ ] d ) are frequently not parallelled in the spine ([Ca 2+ ] s ). This isolation is not caused by a physical diffusion barrier. This report provides, to our knowledge, the first direct demonstration of autonomous spine function.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/354076a0</identifier><identifier>PMID: 1944573</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biochemistry ; Biological and medical sciences ; Calcium ; Calcium - metabolism ; Cellular biology ; Central nervous system ; Central neurotransmission. Neuromudulation. 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It is known that physiological stimuli can cause marked increases in intracellular calcium levels in hippocampal dendritic shafts 12,13 , but it is completely unknown to what extent such changes in the dendrites would also be seen by calcium-sensing structures within spines. Will calcium levels in all spines change in parallel with the dendrite or will there be a heterogeneous response? This study, through direct visualization and measurement of intracellular calcium concentrations in individual living spines, demonstrates that experimentally evoked changes in calcium concentrations in the dendritic shaft ([Ca 2+ ] d ) are frequently not parallelled in the spine ([Ca 2+ ] s ). This isolation is not caused by a physical diffusion barrier. This report provides, to our knowledge, the first direct demonstration of autonomous spine function.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>1944573</pmid><doi>10.1038/354076a0</doi><tpages>5</tpages></addata></record>
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subjects Animals
Biochemistry
Biological and medical sciences
Calcium
Calcium - metabolism
Cellular biology
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Dendrites
Dendrites - physiology
Excavation
Fundamental and applied biological sciences. Psychology
Hippocampus - physiology
Humanities and Social Sciences
In Vitro Techniques
letter
Microscopy, Fluorescence
Minerals
multidisciplinary
Nervous system
Rats
Science
Science (multidisciplinary)
Spine
Synapses - physiology
Vertebrates: nervous system and sense organs
title Independent regulation of calcium revealed by imaging dendritic spines
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