Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-g but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli
Production of periplasmic human interferon-g (hINF-g) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) dri...
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Veröffentlicht in: | Biotechnology letters 2007-09, Vol.29 (9), p.1369-1374 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Production of periplasmic human interferon-g (hINF-g) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-g was processed and the protein was transported to periplasm. Up to 30.1% of hINF-g was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction. |
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ISSN: | 0141-5492 |
DOI: | 10.1007/s10529-007-9395-5 |