Modified penicillin acylase signal peptide allows the periplasmic production of soluble human interferon-g but not of soluble human interleukin-2 by the Tat pathway in Escherichia coli

Production of periplasmic human interferon-g (hINF-g) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) dri...

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Veröffentlicht in:Biotechnology letters 2007-09, Vol.29 (9), p.1369-1374
Hauptverfasser: Medina-Rivero, E, Balderas-Hernandez, V E, Ordonez-Acevedo, L G, Paz-Maldonado, LMT, Barba-De la Rosa, AP, Leon-Rodriguez, A
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Sprache:eng
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Zusammenfassung:Production of periplasmic human interferon-g (hINF-g) and human interleukin-2 (hIL-2) by the Tat translocation pathway in Escherichia coli BL21-SI was evaluated. The expression was obtained using the pEMR vector which contains the Tat-dependent modified penicillin acylase signal peptide (mSPpac) driven by the T7 promoter. The mSPpac-hINF-g was processed and the protein was transported to periplasm. Up to 30.1% of hINF-g was found in the periplasmic soluble fraction, whereas only 15% of the mSPpac-hIL-2 was processed, but hIL-2 was not found in the periplasmic soluble fraction.
ISSN:0141-5492
DOI:10.1007/s10529-007-9395-5