Observations on the vimentin-10-nm filaments during mitosis in BHK21 cells

Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy whic...

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Veröffentlicht in:Experimental cell research 1982-11, Vol.142 (1), p.57-62
Hauptverfasser: Blose, Stephen H., Bushnell, Anne
Format: Artikel
Sprache:eng
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Zusammenfassung:Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy which demonstrated assembled 10-nm filaments in the cytoplasm surrounding the spindle. Therefore, in contrast to previous reports, mitotic BHK21 cells show a similar cage-like 10-nm filament arrangement, as found in a variety of other cell types.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(82)90408-6