Observations on the vimentin-10-nm filaments during mitosis in BHK21 cells
Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy whic...
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Veröffentlicht in: | Experimental cell research 1982-11, Vol.142 (1), p.57-62 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy which demonstrated assembled 10-nm filaments in the cytoplasm surrounding the spindle. Therefore, in contrast to previous reports, mitotic BHK21 cells show a similar cage-like 10-nm filament arrangement, as found in a variety of other cell types. |
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ISSN: | 0014-4827 1090-2422 |
DOI: | 10.1016/0014-4827(82)90408-6 |