Fast screening target sites for RNA interference using a cell-free system

Fast screening target sites for RNA interference using a cell-free system

A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure produces results with an identical reliability compared to those described previously but wh...

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Veröffentlicht in:Biotechnology letters 2005-05, Vol.27 (9), p.619-621
Hauptverfasser: HUANG MEI, JIA, Fang-Jun, LI, Chong-Yong, LI, Yi-Ping, GUO, Li-He
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Biotechnology - methods
Blotting, Northern
Cell-Free System
Cells
Fundamental and applied biological sciences. Psychology
Genetic Techniques
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
Nucleic Acid Hybridization
Plasmids - metabolism
Ribonucleic acid
RNA
RNA Interference
RNA, Small Interfering - metabolism
Temperature
Time Factors
Transfection
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Zusammenfassung:A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure produces results with an identical reliability compared to those described previously but which are more time-consuming than this present method.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-005-3627-3