DNA mutagenesis and recombination

DNA mutagenesis and recombination

The polymerase chain reaction is used for site-specific mutagenesis and for DNA recombination without any enzymatic reaction in vitro, apart from DNA amplification.

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Bibliographische Detailangaben
Veröffentlicht in:Nature (London) 1990-04, Vol.344 (6268), p.793-794
Hauptverfasser: JONES, D. H, SAKAMOTO, K, VORCE, R. L, HOWARD, B. H
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Annealing
Biological and medical sciences
Biotechnology
Cloning
Deoxyribonucleic acid
DNA
DNA, Circular - genetics
DNA, Recombinant
E coli
Enzymes
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Gene Amplification
Gene fusion
Genetic engineering
Genetic technics
Genetics
Methods. Procedures. Technologies
Mutagenesis
Mutation
Phosphorylation
Polymerase Chain Reaction
Primers
Protocol
Recombination
Recombination, Genetic
Single-stranded DNA
Site specific mutagenesis
Transfection
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Beschreibung
Zusammenfassung:The polymerase chain reaction is used for site-specific mutagenesis and for DNA recombination without any enzymatic reaction in vitro, apart from DNA amplification.
ISSN:0028-0836
1476-4687
DOI:10.1038/344793a0