Identification of Virus-Encoded MicroRNAs

RNA silencing processes are guided by small RNAs that are derived from double-stranded RNA. To probe for function of RNA silencing during infection of human cells by a DNA virus, we recorded the small RNA profile of cells infected by Epstein-Barr virus (EBV). We show that EBV expresses several micro...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2004-04, Vol.304 (5671), p.734-736
Hauptverfasser:	Pfeffer, Sébastien, Zavolan, Mihaela, Grässer, Friedrich A., Chien, Minchen, Russo, James J., Ju, Jingyue, John, Bino, Enright, Anton J., Marks, Debora, Sander, Chris, Tuschl, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Callithrix Cell Line Cell Line, Tumor Cell lines Cloning, Molecular Computational Biology Deoxyribonucleic acid DNA DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism DNA-Directed DNA Polymerase - genetics DNA-Directed DNA Polymerase - metabolism Epstein Barr virus infections Epstein-Barr virus Fundamental and applied biological sciences. Psychology Gene Expression Genes Genetic aspects Genetic research Genetics Herpes viruses Herpesvirus 4, Human - genetics Herpesvirus 4, Human - physiology Humans Identification and classification Infections Messenger RNA Microbiology MicroRNA MicroRNAs - genetics MicroRNAs - metabolism Proteins - genetics Proteins - metabolism Ribonucleic acid RNA RNA Interference RNA, Double-Stranded - genetics RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Viral - genetics RNA, Viral - metabolism Transcripts (Written Records) Untranslated Regions Viral Proteins - genetics Viral Proteins - metabolism Virology Virus diseases Virus Latency Viruses
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Beschreibung
Zusammenfassung:	RNA silencing processes are guided by small RNAs that are derived from double-stranded RNA. To probe for function of RNA silencing during infection of human cells by a DNA virus, we recorded the small RNA profile of cells infected by Epstein-Barr virus (EBV). We show that EBV expresses several microRNA (miRNA) genes. Given that miRNAs function in RNA silencing pathways either by targeting messenger RNAs for degradation or by repressing translation, we identified viral regulators of host and/or viral gene expression.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.1096781