Binding of the origin of replication of Escherichia coli to the outer membrane

The replication origin of the Escherichia coli chromosome binds with high affinity to outer membrane preparations. This binding requires a 460 bp stretch of origin DNA between positions −40 and 420 of the oriC map. Specific binding can be detected by the use of a membrane filter retention assay in the presence of excess calf thymus DNA. This binding is enhanced by divalent cations and takes place specifically at a few (0.7-3.0) membrane sites per cell. The apparent affinity of origin DNA for membranes is enhanced by two peptides, (55 kilodaltons (kd) and 75 kd), which remain attached to the DNA through treatment with 5.5 M cesium chloride.