Development of Extraction System by using Thermo-Responsive Polymer for Bovine Serum Albumin Recovery

Thermo-responsive polymers, Dehypon+LS54 and Berol+370 were paired randomly with starch derivatives, K4484+dextrin and N-Lite+D maltodextrin for develop thermoseparating ATPS. The developed systems are DehyponLS54/K4484, DehyponLS54/N-Lite.D and Berol370/K4484. All systems were tested with partitioning of BSA as model protein. Among the tested systems, DehyponLS54/K4484 was found as most suitable system for BSA recovery. A small electrochemical potential effect was observed in DehyponLS54/K4484 dextrin system in which the change of pH (pH 8 to 6) had slightly influence the partitioning behavior of BSA. At both pH, BSA was observed had high affinity to starch enriched phase and it caused a low yield value of BSA after thermoseparation step. However, recovery of BSA in water phase at pH 6 was higher 40% compared to pH 8. The addition of Na(2)SO(4) has lowered the cloud point of Dehypon+LS54.