Induced synthesis of immunoglobulin messenger RNA accompanies induction of immunoglobulin production in cultured mouse spleen cells
Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1978-11, Vol.84 (5), p.1285-1290 |
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| container_end_page | 1290 |
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| container_issue | 5 |
| container_start_page | 1285 |
| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 84 |
| creator | TSUDA, Masa'aki HONJO, Tasuku SHIMIZU, Akira MIZUNO, Den'ichi NATORI, Shunji |
| description | Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The half life of κ chain mRNA was about 9 h. Available evidence indicates that lipopolysaccharide stimulates de novo synthesis of κ chain mRNA. The accumulation of a chain mRNA was markedly suppressed by inhibitors of DNA or protein synthesis such as hydroxyurea, cytosine arabinoside and cycloheximide. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a132247 |
| format | Article |
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(Japan). Faculty of Medicine</creatorcontrib><description>Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The half life of κ chain mRNA was about 9 h. Available evidence indicates that lipopolysaccharide stimulates de novo synthesis of κ chain mRNA. The accumulation of a chain mRNA was markedly suppressed by inhibitors of DNA or protein synthesis such as hydroxyurea, cytosine arabinoside and cycloheximide.</description><identifier>ISSN: 0021-924X</identifier><identifier>EISSN: 1756-2651</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a132247</identifier><identifier>PMID: 103878</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Cycloheximide - pharmacology ; Cytarabine - pharmacology ; Dactinomycin - pharmacology ; DNA - biosynthesis ; Escherichia coli ; Globins - biosynthesis ; Hydroxyurea - pharmacology ; Immunoglobulin kappa-Chains - biosynthesis ; Immunoglobulin Light Chains - biosynthesis ; Lipopolysaccharides - pharmacology ; Male ; Mice ; RNA, Messenger - biosynthesis ; Spleen - cytology ; Uridine - metabolism</subject><ispartof>Journal of biochemistry (Tokyo), 1978-11, Vol.84 (5), p.1285-1290</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c599t-f207ec35bbdb68faa2f0abbf85f4c193aff444cd9b5d5b9284772f48b4916f0f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/103878$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TSUDA, Masa'aki</creatorcontrib><creatorcontrib>HONJO, Tasuku</creatorcontrib><creatorcontrib>SHIMIZU, Akira</creatorcontrib><creatorcontrib>MIZUNO, Den'ichi</creatorcontrib><creatorcontrib>NATORI, Shunji</creatorcontrib><creatorcontrib>Veterinarski Fakultet, Sarajevo (Yugoslavia)</creatorcontrib><creatorcontrib>Tokyo Univ. (Japan). Faculty of Medicine</creatorcontrib><title>Induced synthesis of immunoglobulin messenger RNA accompanies induction of immunoglobulin production in cultured mouse spleen cells</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The half life of κ chain mRNA was about 9 h. Available evidence indicates that lipopolysaccharide stimulates de novo synthesis of κ chain mRNA. The accumulation of a chain mRNA was markedly suppressed by inhibitors of DNA or protein synthesis such as hydroxyurea, cytosine arabinoside and cycloheximide.</description><subject>Animals</subject><subject>Cycloheximide - pharmacology</subject><subject>Cytarabine - pharmacology</subject><subject>Dactinomycin - pharmacology</subject><subject>DNA - biosynthesis</subject><subject>Escherichia coli</subject><subject>Globins - biosynthesis</subject><subject>Hydroxyurea - pharmacology</subject><subject>Immunoglobulin kappa-Chains - biosynthesis</subject><subject>Immunoglobulin Light Chains - biosynthesis</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Male</subject><subject>Mice</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Spleen - cytology</subject><subject>Uridine - metabolism</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptUU1v1DAUtBBfS-EfIJQL3LL4M44PHKqqpYVSEAKx4mLZjr31ktiLnUjtmT-OoywIpJ6e3puZ98YeAF4iuEZQkNfxxsXU7eKUgurzeqfNtR3WChGMKb8HVoizpsYNQ_fBCkKMaoHp5jF4kvNubjEhj8BDBEnL2xX4dRG6ydiuyrdhvLbZ5yq6yg_DFOK2j3rqfagGm7MNW5uqz1fHlTImDnsVvM2Vn9Wjj-EO1T7FP2DpzNSPUyqHhjhlW-V9b22Z2r7PT8EDV15inx3qEfh6dvrl5Ly-_Pj24uT4sjZMiLF2GHJrCNO6003rlMIOKq1dyxw1SBDlHKXUdEKzjmmBW8o5drTVVKDGQUeOwKtlb3H2c7J5lIPPswMVbDElOSUQMtgU4puFaFLMOVkn98kPKt1KBOWcgfw_A7lkIA8ZFP3zw6FJD7b7Rz1_eoHrBfZ5tDd_UZV-yIYTzuT55rv8sHlPyDeE5abwXyx8p6JU2-SzfPcJCS4gRaSsJL8BUcKn9Q</recordid><startdate>197811</startdate><enddate>197811</enddate><creator>TSUDA, Masa'aki</creator><creator>HONJO, Tasuku</creator><creator>SHIMIZU, Akira</creator><creator>MIZUNO, Den'ichi</creator><creator>NATORI, Shunji</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197811</creationdate><title>Induced synthesis of immunoglobulin messenger RNA accompanies induction of immunoglobulin production in cultured mouse spleen cells</title><author>TSUDA, Masa'aki ; HONJO, Tasuku ; SHIMIZU, Akira ; MIZUNO, Den'ichi ; NATORI, Shunji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c599t-f207ec35bbdb68faa2f0abbf85f4c193aff444cd9b5d5b9284772f48b4916f0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Animals</topic><topic>Cycloheximide - pharmacology</topic><topic>Cytarabine - pharmacology</topic><topic>Dactinomycin - pharmacology</topic><topic>DNA - biosynthesis</topic><topic>Escherichia coli</topic><topic>Globins - biosynthesis</topic><topic>Hydroxyurea - pharmacology</topic><topic>Immunoglobulin kappa-Chains - biosynthesis</topic><topic>Immunoglobulin Light Chains - biosynthesis</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Male</topic><topic>Mice</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Spleen - cytology</topic><topic>Uridine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TSUDA, Masa'aki</creatorcontrib><creatorcontrib>HONJO, Tasuku</creatorcontrib><creatorcontrib>SHIMIZU, Akira</creatorcontrib><creatorcontrib>MIZUNO, Den'ichi</creatorcontrib><creatorcontrib>NATORI, Shunji</creatorcontrib><creatorcontrib>Veterinarski Fakultet, Sarajevo (Yugoslavia)</creatorcontrib><creatorcontrib>Tokyo Univ. (Japan). Faculty of Medicine</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TSUDA, Masa'aki</au><au>HONJO, Tasuku</au><au>SHIMIZU, Akira</au><au>MIZUNO, Den'ichi</au><au>NATORI, Shunji</au><aucorp>Veterinarski Fakultet, Sarajevo (Yugoslavia)</aucorp><aucorp>Tokyo Univ. (Japan). Faculty of Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induced synthesis of immunoglobulin messenger RNA accompanies induction of immunoglobulin production in cultured mouse spleen cells</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1978-11</date><risdate>1978</risdate><volume>84</volume><issue>5</issue><spage>1285</spage><epage>1290</epage><pages>1285-1290</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><abstract>Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The half life of κ chain mRNA was about 9 h. Available evidence indicates that lipopolysaccharide stimulates de novo synthesis of κ chain mRNA. The accumulation of a chain mRNA was markedly suppressed by inhibitors of DNA or protein synthesis such as hydroxyurea, cytosine arabinoside and cycloheximide.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>103878</pmid><doi>10.1093/oxfordjournals.jbchem.a132247</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of biochemistry (Tokyo), 1978-11, Vol.84 (5), p.1285-1290 |
| issn | 0021-924X 1756-2651 |
| language | eng |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals Digital Archive Legacy; Free Full-Text Journals in Chemistry |
| subjects | Animals Cycloheximide - pharmacology Cytarabine - pharmacology Dactinomycin - pharmacology DNA - biosynthesis Escherichia coli Globins - biosynthesis Hydroxyurea - pharmacology Immunoglobulin kappa-Chains - biosynthesis Immunoglobulin Light Chains - biosynthesis Lipopolysaccharides - pharmacology Male Mice RNA, Messenger - biosynthesis Spleen - cytology Uridine - metabolism |
| title | Induced synthesis of immunoglobulin messenger RNA accompanies induction of immunoglobulin production in cultured mouse spleen cells |
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