Induced synthesis of immunoglobulin messenger RNA accompanies induction of immunoglobulin production in cultured mouse spleen cells

Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 1978-11, Vol.84 (5), p.1285-1290
Hauptverfasser:	TSUDA, Masa'aki, HONJO, Tasuku, SHIMIZU, Akira, MIZUNO, Den'ichi, NATORI, Shunji
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cycloheximide - pharmacology Cytarabine - pharmacology Dactinomycin - pharmacology DNA - biosynthesis Escherichia coli Globins - biosynthesis Hydroxyurea - pharmacology Immunoglobulin kappa-Chains - biosynthesis Immunoglobulin Light Chains - biosynthesis Lipopolysaccharides - pharmacology Male Mice RNA, Messenger - biosynthesis Spleen - cytology Uridine - metabolism
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Zusammenfassung:	Immunoglobulin κ type light chain mRNA (Lκ mRNA) accumulated in parallel with secretion of immunoglobulin M in cultured mouse spleen cells activated by lipopolysaccharide. Actinomycin D suppressed the accumulation of κ chain mRNA completely without affecting the degradation rate of κ chain mRNA. The half life of κ chain mRNA was about 9 h. Available evidence indicates that lipopolysaccharide stimulates de novo synthesis of κ chain mRNA. The accumulation of a chain mRNA was markedly suppressed by inhibitors of DNA or protein synthesis such as hydroxyurea, cytosine arabinoside and cycloheximide.
ISSN:	0021-924X 1756-2651
DOI:	10.1093/oxfordjournals.jbchem.a132247