Peroxide-induced conformational transitions of peanut storage protein (arachin)

Several effects of peroxides on protein have been described in the literature. Some of the effects have been ascribed to alterations in protein conformation but this has never been examined directly. This paper describes responses of peanut storage globulin (arachin) to rancid oil and hydrogen perox...

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Veröffentlicht in:International Journal of Peptide and Protein Research 1982-08, Vol.20 (2), p.149-153
Hauptverfasser: JACKS, T.J., HENSARLING, T.P., MULLER, L.L., ANGELO, A.J. ST, NEUCERE, N.J.
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Sprache:eng
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Zusammenfassung:Several effects of peroxides on protein have been described in the literature. Some of the effects have been ascribed to alterations in protein conformation but this has never been examined directly. This paper describes responses of peanut storage globulin (arachin) to rancid oil and hydrogen peroxide as measured by electrophoretic mobility, antigenicity and circular dichroism of the globulin. Rancid oil and 3% hydrogen peroxide had little effect on these properties but 30% hydrogen peroxide increased the mobility, abolished the antigenicity and reduced the ordered structural modes of the protein. The results indicated that changes in protein conformation do not occur after contact with organoleptically rancid oil or dilute peroxides but do occur with concentrated peroxides.
ISSN:0367-8377
1399-3011
DOI:10.1111/j.1399-3011.1982.tb02668.x