Separation and identification of subpicomole amounts of methotrexate polyglutamates in animal and human biopsy material

A rapid, quantitative procedure for separating methotrexate (MTX) polyglutamates utilizing molecular-sieve column chromatography has been developed. MTX polyglutamates were separated in 25 min and detected by a radioassay with a sensitivity of 0.1 pmol MTX, allowing analysis on 2 to 5 mg of tissue....

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Veröffentlicht in:	Analytical biochemistry 1982-05, Vol.122 (2), p.412-416
Hauptverfasser:	Krakower, Glenn R., Nylen, Patricia A., Kamen, Barton A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain Chemistry Chromatography, High Pressure Liquid Humans Kidney - analysis Liver - analysis Methotrexate - analogs & derivatives Methotrexate - analysis Microchemistry Polyglutamic Acid - analogs & derivatives Polyglutamic Acid - analysis Radioligand Assay Rats Tissue Distribution
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Beschreibung
Zusammenfassung:	A rapid, quantitative procedure for separating methotrexate (MTX) polyglutamates utilizing molecular-sieve column chromatography has been developed. MTX polyglutamates were separated in 25 min and detected by a radioassay with a sensitivity of 0.1 pmol MTX, allowing analysis on 2 to 5 mg of tissue. This technique has been used to demonstrate and quantitate the levels of MTX polyglutamates in liver, kidney, and brain tissues of rats treated with weekly low doses of MTX, and in liver and red cells of patients with acute leukemia treated with weekly low doses of MTX.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(82)90303-7