Effect of pH and other conditions on immunosorbent electron microscopy of several plant viruses

The efficiency of trapping, using immunosorbent electron microscopy, of particles of cucumber mosaic virus, lily symptomless virus, potato virus Y, and carnation mottle virus, depended markedly on the pH of the virus extract. Varying the pH of the serum had a smaller, though still significant effect. With potato virus Y, the optimal pH of the virus extract varied with the host plant. It appears that for each virus and host a specific testing procedure is required to find the optimal trapping conditions.