Plasminogen activator and MIF-like activities in Kirsten virus transformed mouse NIH culture fluids

Plasminogen activator and MIF-like activities in Kirsten virus transformed mouse NIH culture fluids

Kirsten virus transformed mouse NIH cells produce both a macrophage migration inhibition activity for guinea pig and mouse peritoneal exudate cells and a plasminogen activator. The migration inhibition factor activity exhibited thermal stability up to 80°C while the plasminogen activator was inactiv...

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Veröffentlicht in:Biochemical and biophysical research communications 1978-08, Vol.83 (3), p.1164-1170
Hauptverfasser: Feder, J., Kimes, R.C., Tolbert, W.R., Cleveland, C., Hammond, M.E., Orenstein, N.S., Goodwin, J., Dvorak, H.F.
Format: Artikel
Sprache:eng
Schlagworte:
Cell Line
Cell Transformation, Viral
Culture Media
Drug Stability
Macrophage Migration-Inhibitory Factors - isolation & purification
Macrophage Migration-Inhibitory Factors - pharmacology
Macrophages - drug effects
Plasminogen Activators - isolation & purification
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Zusammenfassung:Kirsten virus transformed mouse NIH cells produce both a macrophage migration inhibition activity for guinea pig and mouse peritoneal exudate cells and a plasminogen activator. The migration inhibition factor activity exhibited thermal stability up to 80°C while the plasminogen activator was inactivated after 15 minutes at 70°C. Separation of these activities was achieved by absorption of the migration inhibition activity on agarose-fucosamine or high speed centrifugation.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(78)91517-6