GASTROINTESTINAL DISORDERS DUE TO INCREASED BLOOD COAGULATION: II. Role of Tissue Fibrinolysis and Lysosomal Enzymes in an Experimental DIC (Disseminated Intravascular Coagulation) of dogs

The role of tissue fibrinolysis and lysosomal enzymes on the hemorrhagic necrosis of digestive mucosa was investigated in an experimental model of DIC (disseminated intravascular coagulation), produced by intravenous administration of bacterial lipopolysaccharide (endotoxin) in dogs. The fibrinolytic activity in the biopsied digestive mucosa revealed marked elevation following the administration of endotoxin, to which the changes of the lysosomal enzymes such as acid phosphatase and beta-glucuronidase were found to be correlated. Pretreatment of the intestinal mucosa of dogs by trans-AMCHA, or by Trasylol, prevented the intestinal mucosa from hemorrhagic necrosis in DIC. The lysosomal enzymes were markedly reduced at the mucosa pretreated by these chemicals, suggesting the possibility that the release of lysosomal enzymes from the mucosal cells were inhibited, or the activity of lysosomal enzymes released were inhibited by either trans-AMCHA or Trasylol. These data led us to conclude that the lysosomal enzymes, including plasminogen activator, play an important role in the pathogenesis of hemorrhagic necrosis associated with DIC.