A quantitative ELISA for IgG in cell culture supernatants

The measurement of IgG, using a sandwich-type enzyme-linked immunosorbent assay (ELISA), is described. The assay is specific, sensitive, and simple to perform. Purification of the antibody, prior to attachment to the plastic solid phase, results in a more sensitive test. Optimum standardisation of t...

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Veröffentlicht in:	Clinica chimica acta 1982-08, Vol.123 (3), p.303-310
Hauptverfasser:	Wakefield, Elizabeth F., Shelton, Michael J., Hosking, Clifford S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - analysis Antibody Affinity Blood Cells, Cultured Culture Media - analysis Enzyme-Linked Immunosorbent Assay Humans Immunoenzyme Techniques Immunoglobulin G - analysis Immunoglobulin G - immunology
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	The measurement of IgG, using a sandwich-type enzyme-linked immunosorbent assay (ELISA), is described. The assay is specific, sensitive, and simple to perform. Purification of the antibody, prior to attachment to the plastic solid phase, results in a more sensitive test. Optimum standardisation of the reagents and conditions is described and discussed. The assay is capable of detecting IgG to 2.5 × 10 −5 IU/ml (≈ 3 ng/ml) and is useful for measuring IgG produced by cells in culture. The coefficient of variation within assays is 6.0% and between assays is 7.7%.
ISSN:	0009-8981 1873-3492
DOI:	10.1016/0009-8981(82)90175-9