Purification from cultured hepatoma cells of two nonhistone chromatin proteins with preferential affinity for single-stranded DNA: Apparent analogy with calf thymus HMG proteins

Purification from cultured hepatoma cells of two nonhistone chromatin proteins with preferential affinity for single-stranded DNA: Apparent analogy with calf thymus HMG proteins

Sequential chromatography on double-stranded DNA and single-stranded DNA columns selects two proteins with marked preference for single-stranded DNA from the complex set of proteins that is released by NaCl from chromatin of cultured hepatoma cells. By a number of criteria, these two proteins appear...

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Veröffentlicht in:Biochemical and biophysical research communications 1978-12, Vol.85 (3), p.1211-1218
Hauptverfasser: Bidney, Dennis L., Reeck, Gerald R.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acids - analysis
animal science
Animals
Carcinoma, Hepatocellular - analysis
Cattle
Cell Line
Chromatin - analysis
Chromatography, Affinity
Chromosomal Proteins, Non-Histone - isolation & purification
DNA
DNA, Single-Stranded
Liver Neoplasms
livestock
Nucleoproteins
Thymus Gland
zoology
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Zusammenfassung:Sequential chromatography on double-stranded DNA and single-stranded DNA columns selects two proteins with marked preference for single-stranded DNA from the complex set of proteins that is released by NaCl from chromatin of cultured hepatoma cells. By a number of criteria, these two proteins appear to be analogous to the calf thymus chromatin proteins HMG-1 and HMG-2.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(78)90671-X