Neuronal Mapping: A Photooxidation Reaction Makes Lucifer Yellow Useful for Electron Microscopy

Neuronal Mapping: A Photooxidation Reaction Makes Lucifer Yellow Useful for Electron Microscopy

Irradiating Lucifer yellow-filled neurons with intense blue light in the presence of 3,3$^{\prime}$-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second i...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1982-09, Vol.217 (4563), p.953-955
1. Verfasser: Maranto, Anthony R.
Format: Artikel
Sprache:eng
Schlagworte:
3,3'-Diaminobenzidine
Animals
Astacoidea
Dyes
Electron microscopy
Electrons
Fluorescence
Ganglia
Ganglia - physiology
Ganglia - ultrastructure
In Vitro Techniques
Isoquinolines
Leeches
Light
Microscopes
Microscopy
Microscopy, Electron - methods
Neurons
Neurons - physiology
Neurons - ultrastructure
Product labeling
Reaction products
Synapses - ultrastructure
Tracers (Biology)
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Zusammenfassung:Irradiating Lucifer yellow-filled neurons with intense blue light in the presence of 3,3$^{\prime}$-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.7112109