Manganese and calcium binding sites of concanavalin A
The atomic co-ordinates of concanavalin A, a lectin which specifically binds saccharides containing mannosyl and glucosyl residues, have been refined with the fast Fourier least-squares method of Agarwal (1978), with X-ray crystallographic data to 1.75 Å. This metalloprotein contains six co-ordinate...
Gespeichert in:
Veröffentlicht in: | Journal of molecular biology 1982-01, Vol.157 (1), p.69-86 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The atomic co-ordinates of concanavalin A, a lectin which specifically binds saccharides containing mannosyl and glucosyl residues, have been refined with the fast Fourier least-squares method of Agarwal (1978), with X-ray crystallographic data to 1.75 Å. This metalloprotein contains six co-ordinated Mn
2+ and seven co-ordinated Ca
2+ which are 4.25 Å apart and are close to the carbohydrate binding site. Both ions are pseudo-octahedral. This is possible for calcium since the average position of two oxygens (from the same carboxyl group) forms one apex of the octahedron. The refinement has improved this region considerably. The ligands for Mn
2+ are five oxygens, three from carboxyl side-chains and two from water molecules, and one nitrogen from a histidyl side-chain. The co-ordination sphere for Ca
2+ contains seven oxygens, three from two carboxyl side-chains, one from a peptide carbonyl, one from an amide side-chain and, as does Mn
2+, two from water molecules. The
averages for the five Mn
2+O and the seven Ca
2+O bonds are 2.28 ± 0.04 (standard deviation) and 2.45 ± 0.09, respectively. For Mn
2+, this
average is 0.10 Å higher than that found for the Mn(II)hexa-aquoion and is toward the high end of the range usually found for Mn
2+O complexes. This is of particular significance since Mn
2+ is used as a probe for nuclear magnetic resonance spectroscopic studies of native Mn
2+- and Mn
2+-substituted metalloproteins. Also, refinement confirms the non-proline
cis peptide bond, which is immediately adjacent to the Ca
2+ and the carbohydrate binding sites. For the final model, the bond lengths and angles for backbone atoms have root-mean-square deviations from standard values of 0.030 Å and 5.2 deg., and side-chain atoms have slightly lower values. The crystallographic residual,
R factor, for this model is 0.167 for 22,042 structure factors. |
---|---|
ISSN: | 0022-2836 1089-8638 |
DOI: | 10.1016/0022-2836(82)90513-7 |