Light-induced free-radical reactions of purines and pyrimidines in deoxyribonucleic acid. Effect of structure and base sequence on reactivity

In the present, paper the authors describe the reactivities of purines and pyrimidines in the various forms of Phi X174 DNA in the light-induced free-radical alkylation reactions with 2-propanol and evaluate the effect of the secondary and the tertiary structure of the DNA on the reactivity of the bases. Evidence is presented for sequence selectivity of these reactions in deoxyribonucleoside monophosphates, ordered synthetic deoxyribopolynucleotides, and Phi X174 DNA.