CONVENIENT PURIFICATION OF HUMAN SPLEEN FIBRINOLYTIC PROTEINASE (SFP) AND HUMAN LEUCOCYTE ELASTASE BY AFFINITY CHROMATOGRAPHY

A peptide inhibitor, Suc-L-Tyr-D-Leu-D-Val-pNA, for human spleen fibrinolytic proteinase (SFP) and for human leucocyte elastase was coupled with AH Sepharose 4B using water soluble carbodiimide. A 116-fold purification of the crude extract of SFP and a 59-fold purification of the partially purified...

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Veröffentlicht in:	Chemical & pharmaceutical bulletin 1982/04/25, Vol.30(4), pp.1528-1530
Hauptverfasser:	Okada, Yoshio, Tsuda, Yuko, Nagamatsu, Yoko, Okamoto, Utako
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Affinity - methods Endopeptidases - isolation & purification Humans In Vitro Techniques leukocyte elastase leukocytes Leukocytes - enzymology man Pancreatic Elastase - isolation & purification proteinase purification of enzyme spleen Spleen - enzymology
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Zusammenfassung:	A peptide inhibitor, Suc-L-Tyr-D-Leu-D-Val-pNA, for human spleen fibrinolytic proteinase (SFP) and for human leucocyte elastase was coupled with AH Sepharose 4B using water soluble carbodiimide. A 116-fold purification of the crude extract of SFP and a 59-fold purification of the partially purified leucocyte elastase were achieved by (Suc-L-Tyr-D-Leu-D-Val-pNA)-AH Sepharose 4B affinity chromatography.
ISSN:	0009-2363 1347-5223
DOI:	10.1248/cpb.30.1528