High yield preparation of isolated human adult hepatocytes by enzymatic perfusion of the liver

High yield preparation of isolated human adult hepatocytes by enzymatic perfusion of the liver

We have developed experimental conditions allowing the isolation of as many as 1–10 × 10 8 human adult hepatocytes by perfusing the left lobe of the liver. About 70–85% cells were viable with the trypan blue exclusion test; they attached to substrates and formed monolayers which survived for up to 8...

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Veröffentlicht in:Cell biology international reports 1982-06, Vol.6 (6), p.625-628
Hauptverfasser: Guguen-Guillouzo, C., Campion, J.P., Brissot, P., Glaise, D., Launois, B., Bourel, M., Guillouzo, A.
Format: Artikel
Sprache:eng
Schlagworte:
Adult
Animals
Cell Separation
Cell Survival
Cells, Cultured
Humans
Liver - cytology
Liver - ultrastructure
Microbial Collagenase
Microscopy, Electron
Microscopy, Phase-Contrast
Papio
Perfusion
Rats
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Zusammenfassung:We have developed experimental conditions allowing the isolation of as many as 1–10 × 10 8 human adult hepatocytes by perfusing the left lobe of the liver. About 70–85% cells were viable with the trypan blue exclusion test; they attached to substrates and formed monolayers which survived for up to 8 days in culture.
ISSN:0309-1651
1878-240X
DOI:10.1016/0309-1651(82)90187-4