Precise structural information for transient enzyme-substrate complexes by a combined x-ray crystallographic-resonance Raman spectroscopic approach

Precise structural information for transient enzyme-substrate complexes by a combined x-ray crystallographic-resonance Raman spectroscopic approach

A combined resonance Raman (RR) spectroscopic and X-ray crystallographic analysis of single crystals of dithioester model compounds is used to set up precise structure-spectra correlations. In turn, these correlations allow the authors to obtain detailed information on the acyl group structure in so...

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Veröffentlicht in:Biochemistry (Easton) 1982-06, Vol.21 (13), p.3109-3115
Hauptverfasser: Huber, C. P, Ozaki, Y, Pliura, D. H, Storer, A. C, Carey, P. R
Format: Artikel
Sprache:eng
Schlagworte:
active sites
Binding Sites
Enzymes - metabolism
Molecular Conformation
papain
Papain - metabolism
Protein Binding
Protein Conformation
Raman spectroscopy
Spectrum Analysis, Raman
X-ray crystallography
X-Ray Diffraction
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Zusammenfassung:A combined resonance Raman (RR) spectroscopic and X-ray crystallographic analysis of single crystals of dithioester model compounds is used to set up precise structure-spectra correlations. In turn, these correlations allow the authors to obtain detailed information on the acyl group structure in some acylpapain intermediates.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00256a012