Analysis of T Lymphocytes After Bone Marrow Transplantation Using Monoclonal Antibodies

Using monoclonal antibodies to cell surface antigens and fluorescent cell sorter analysis, we studied peripheral blood lymphocyte subsets after bone marrow transplantation (BMT). In 13 patients studied 3 mo or more after BMT, the ratio of T-cell subsets defined by antibodies OKT4 and OKT8 was revers...

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Veröffentlicht in:Blood 1982-09, Vol.60 (3), p.578-582
Hauptverfasser: Fox, Robert, McMillan, Robert, Spruce, Wayne, Tani, Patti, Mason, Donna
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Sprache:eng
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Zusammenfassung:Using monoclonal antibodies to cell surface antigens and fluorescent cell sorter analysis, we studied peripheral blood lymphocyte subsets after bone marrow transplantation (BMT). In 13 patients studied 3 mo or more after BMT, the ratio of T-cell subsets defined by antibodies OKT4 and OKT8 was reversed (OKT4/OKT8 = 0.7 ± 0.3) in comparison to normal volunteers or bone marrow donors (ratio OKT4/OKT8 = 1.7 ± 0.4) (p < 0.001). This reversed ratio persisted for up to 3 yr after BMT. In contrast to a previous report, the presence of an abnormal ratio of T-cell subsets did not correlate with clinically significant graft-versushost disease (GVHD). In agreement with a previous study, we found increased la-positive T cells in BMT patients (26% ± 8%;
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V60.3.578.578