A Simple Method for the Determination of Glucosamine and Galactosamine Using Cellulose Acetate Electrophoresis

A Simple Method for the Determination of Glucosamine and Galactosamine Using Cellulose Acetate Electrophoresis

A new electrophoretic method is presented for the determination of glucosamine and galactosamine. The technique is quite simple and rapid, it involves cellulose acetate electrophoresis in borate buffer (200 V, 15 min) and silver nitrate staining. Hexosamine samples of 0.36-1.80 μg were separated and...

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Veröffentlicht in:Journal of biochemistry (Tokyo) 1982-04, Vol.91 (4), p.1445-1447
Hauptverfasser: MIYAMOTO, Itaru, NAGASE, Sumi
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Electrophoresis, Cellulose Acetate - methods
Galactosamine - analysis
Glucosamine - analysis
In Vitro Techniques
Mice
Mice, Inbred ICR
Rats
Rats, Inbred Strains
Sharks
Skin - analysis
Swine
Whales
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Beschreibung
Zusammenfassung:A new electrophoretic method is presented for the determination of glucosamine and galactosamine. The technique is quite simple and rapid, it involves cellulose acetate electrophoresis in borate buffer (200 V, 15 min) and silver nitrate staining. Hexosamine samples of 0.36-1.80 μg were separated and stained within 30 min. This method was applied for the hexosamine analysis of glycosaminoglycans.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a133834