Conversion of free 3β-hydroxy-5-ene-steroids by incubation with Helix pomatia
The incubation of the 3β-hydroxy-5-ene-steroids, pregnenolone, 5-pregnene-3β,20a-diol and 17-hydroxypregnenolone with an enzyme preparation from Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids wer...
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| Veröffentlicht in: | Journal of steroid biochemistry 1982-05, Vol.16 (5), p.701-703 |
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| container_end_page | 703 |
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| container_issue | 5 |
| container_start_page | 701 |
| container_title | Journal of steroid biochemistry |
| container_volume | 16 |
| creator | Vanluchene, E. Eechaute, W. Vandekerckhove, D. |
| description | The incubation of the 3β-hydroxy-5-ene-steroids, pregnenolone, 5-pregnene-3β,20a-diol and 17-hydroxypregnenolone with an enzyme preparation from
Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids were identified. Therefore, when using
Helix pomatia extract the measurement of both 3β-hydroxy-5-ene-and 4-ene-3-oxo-steroids in biological fluids may be unreliable. |
| doi_str_mv | 10.1016/0022-4731(82)90107-8 |
| format | Article |
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Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids were identified. Therefore, when using
Helix pomatia extract the measurement of both 3β-hydroxy-5-ene-and 4-ene-3-oxo-steroids in biological fluids may be unreliable.</description><identifier>ISSN: 0022-4731</identifier><identifier>DOI: 10.1016/0022-4731(82)90107-8</identifier><identifier>PMID: 7098484</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>17-alpha-Hydroxypregnenolone - metabolism ; Animals ; Chromatography, Gas ; Helix (Snails) - metabolism ; Hydroxycorticosteroids - metabolism ; Pregnanolone - metabolism ; Pregnenolone - analogs & derivatives ; Pregnenolone - metabolism</subject><ispartof>Journal of steroid biochemistry, 1982-05, Vol.16 (5), p.701-703</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-ea34be97f5543346dac64cf343f3fc4729082136720b73c3b97d3bc226497003</citedby><cites>FETCH-LOGICAL-c357t-ea34be97f5543346dac64cf343f3fc4729082136720b73c3b97d3bc226497003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7098484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vanluchene, E.</creatorcontrib><creatorcontrib>Eechaute, W.</creatorcontrib><creatorcontrib>Vandekerckhove, D.</creatorcontrib><title>Conversion of free 3β-hydroxy-5-ene-steroids by incubation with Helix pomatia</title><title>Journal of steroid biochemistry</title><addtitle>J Steroid Biochem</addtitle><description>The incubation of the 3β-hydroxy-5-ene-steroids, pregnenolone, 5-pregnene-3β,20a-diol and 17-hydroxypregnenolone with an enzyme preparation from
Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids were identified. Therefore, when using
Helix pomatia extract the measurement of both 3β-hydroxy-5-ene-and 4-ene-3-oxo-steroids in biological fluids may be unreliable.</description><subject>17-alpha-Hydroxypregnenolone - metabolism</subject><subject>Animals</subject><subject>Chromatography, Gas</subject><subject>Helix (Snails) - metabolism</subject><subject>Hydroxycorticosteroids - metabolism</subject><subject>Pregnanolone - metabolism</subject><subject>Pregnenolone - analogs & derivatives</subject><subject>Pregnenolone - metabolism</subject><issn>0022-4731</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1OwzAQhb0AlVK4AUhZIVgY_Jc42SChCihSBZvurcQZq0ZJXOwEmmtxEM5EQqsuWc3ozbw3mg-hC0puKaHJHSGMYSE5vU7ZTUYokTg9QtODfIJOQ3gnhGapYBM0kWRoUjFFr3PXfIIP1jWRM5HxABH_-cbrvvRu2-MYQwM4tOCdLUNU9JFtdFfk7Wj4su06WkBlt9HG1YOWn6Fjk1cBzvd1hlZPj6v5Ai_fnl_mD0useSxbDDkXBWTSxLHgXCRlrhOhDRfccKOFZBlJGeWJZKSQXPMikyUvNGOJyCQhfIaudrEb7z46CK2qbdBQVXkDrgtKCsozmmTDotgtau9C8GDUxts6972iRI3k1IhIjYhUytQfOZUOtst9flfUUB5Me2zD_H43h-HHTwteBW2h0VBaD7pVpbP_H_gF9m1-pw</recordid><startdate>198205</startdate><enddate>198205</enddate><creator>Vanluchene, E.</creator><creator>Eechaute, W.</creator><creator>Vandekerckhove, D.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198205</creationdate><title>Conversion of free 3β-hydroxy-5-ene-steroids by incubation with Helix pomatia</title><author>Vanluchene, E. ; Eechaute, W. ; Vandekerckhove, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-ea34be97f5543346dac64cf343f3fc4729082136720b73c3b97d3bc226497003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>17-alpha-Hydroxypregnenolone - metabolism</topic><topic>Animals</topic><topic>Chromatography, Gas</topic><topic>Helix (Snails) - metabolism</topic><topic>Hydroxycorticosteroids - metabolism</topic><topic>Pregnanolone - metabolism</topic><topic>Pregnenolone - analogs & derivatives</topic><topic>Pregnenolone - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vanluchene, E.</creatorcontrib><creatorcontrib>Eechaute, W.</creatorcontrib><creatorcontrib>Vandekerckhove, D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of steroid biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vanluchene, E.</au><au>Eechaute, W.</au><au>Vandekerckhove, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conversion of free 3β-hydroxy-5-ene-steroids by incubation with Helix pomatia</atitle><jtitle>Journal of steroid biochemistry</jtitle><addtitle>J Steroid Biochem</addtitle><date>1982-05</date><risdate>1982</risdate><volume>16</volume><issue>5</issue><spage>701</spage><epage>703</epage><pages>701-703</pages><issn>0022-4731</issn><abstract>The incubation of the 3β-hydroxy-5-ene-steroids, pregnenolone, 5-pregnene-3β,20a-diol and 17-hydroxypregnenolone with an enzyme preparation from
Helix pomatia used to hydrolyse steroid conjugates, may lead to changed structures. Using capillary gas chromatography the corresponding 4-ene-steroids were identified. Therefore, when using
Helix pomatia extract the measurement of both 3β-hydroxy-5-ene-and 4-ene-3-oxo-steroids in biological fluids may be unreliable.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>7098484</pmid><doi>10.1016/0022-4731(82)90107-8</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-4731 |
| ispartof | Journal of steroid biochemistry, 1982-05, Vol.16 (5), p.701-703 |
| issn | 0022-4731 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_74139169 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 17-alpha-Hydroxypregnenolone - metabolism Animals Chromatography, Gas Helix (Snails) - metabolism Hydroxycorticosteroids - metabolism Pregnanolone - metabolism Pregnenolone - analogs & derivatives Pregnenolone - metabolism |
| title | Conversion of free 3β-hydroxy-5-ene-steroids by incubation with Helix pomatia |
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